The Mouse MBP (Myelin Basic Protein) ELISA Kit is a reliable and sensitive assay designed for the detection of MBP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, ensuring accurate and reproducible results for a variety of research applications.Myelin Basic Protein (MBP) is a key component of the myelin sheath in the nervous system and plays a crucial role in the proper functioning of nerve cells. Changes in MBP levels have been associated with various neurological disorders such as multiple sclerosis and other demyelinating diseases, making it an important biomarker for studying these conditions and potential therapeutic interventions.
Overall, the Mouse MBP ELISA Kit provides researchers with a valuable tool for studying the role of MBP in neurodegenerative disorders and other related conditions, leading to a better understanding of disease mechanisms and potential treatment options.
Product Name:
Mouse MBP (Myelin Basic Protein) ELISA Kit
SKU:
MOES01280
Target:
Mouse MBP (Myelin Basic Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MBP. You can calculate the concentration of Mouse MBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
97-115
93-108
Average (%)
91
105
100
1:4
Range (%)
88-105
87-99
86-100
Average (%)
96
92
91
1:8
Range (%)
91-104
85-98
85-97
Average (%)
96
92
90
1:16
Range (%)
90-100
83-93
86-97
Average (%)
95
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
0.92
3.7
0.54
0.95
4.02
Standard deviation
0.03
0.05
0.18
0.03
0.04
0.13
C V (%)
5.56
5.43
4.86
5.56
4.21
3.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MBP in samples. No significant cross-reactivity or interference between Mouse MBP and analogues was observed.
