The Mouse MAU (Microalbuminuria) ELISA Kit is specifically designed for the precise measurement of microalbuminuria levels in mouse serum, urine, and other biological samples. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Microalbuminuria is a key indicator of renal dysfunction and early kidney damage, serving as a valuable biomarker for monitoring kidney health in various disease states.
With the Mouse MAU ELISA Kit, researchers can confidently study renal function and explore potential therapeutic strategies for kidney-related disorders with ease.Overall, the Mouse MAU ELISA Kit is a reliable tool for investigating kidney function in murine models, offering valuable insights into the mechanisms underlying renal diseases and facilitating the development of novel treatment approaches.
Product Name:
Mouse MAU (Microalbuminuria) ELISA Kit
SKU:
MOES01268
Target:
Mouse MAU (Microalbuminuria)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MAU. You can calculate the concentration of Mouse MAU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-104
94-107
87-99
Average (%)
99
102
93
1:4
Range (%)
92-103
97-106
89-98
Average (%)
99
101
95
1:8
Range (%)
101-107
97-109
97-107
Average (%)
104
105
100
1:16
Range (%)
89-104
89-97
87-100
Average (%)
96
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
86-96
90
Cell culture media (n=5)
100-107
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.45
1.17
4.29
0.48
1.13
4.64
Standard deviation
0.02
0.06
0.22
0.03
0.05
0.22
C V (%)
4.44
5.13
5.13
6.25
4.42
4.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MAU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MAU in samples. No significant cross-reactivity or interference between Mouse MAU and analogues was observed.
