The Mouse LTF (LF Lactoferrin) ELISA Kit is specifically designed for the precise measurement of LF Lactoferrin levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring dependable and consistent results, thereby making it an excellent choice for a variety of research applications.LF Lactoferrin is a key protein known for its antimicrobial and immunomodulatory properties.
It plays a crucial role in the immune response, inflammation, and iron metabolism, making it a valuable biomarker for studying various health conditions and potential therapeutic avenues.With easy-to-follow protocols and rapid assay procedures, the Mouse LTF (LF Lactoferrin) ELISA Kit provides researchers with a highly efficient tool for investigating the role of LF Lactoferrin in different biological processes and diseases.
Product Name:
Mouse LTF/LF (Lactoferrin) ELISA Kit
SKU:
MOES01235
Target:
Mouse LTF/LF (Lactoferrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LTF/LF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LTF/LF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LTF/LF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LTF/LF. You can calculate the concentration of Mouse LTF/LF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
93-106
84-98
Average (%)
96
99
91
1:4
Range (%)
94-111
79-92
93-110
Average (%)
102
86
101
1:8
Range (%)
100-115
83-95
93-110
Average (%)
107
89
101
1:16
Range (%)
95-110
85-96
99-115
Average (%)
100
90
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
97
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.5
360.0
1557.8
188.1
334.2
1664.8
Standard deviation
11.2
15.8
57.6
10.9
13.4
88.2
C V (%)
5.82
4.39
3.7
5.79
4.01
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LTF/LF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LTF/LF in samples. No significant cross-reactivity or interference between Mouse LTF/LF and analogues was observed.
