Mouse LGMN Recombinant Protein (RPPB1903)

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SKU:
RPPB1903
Product type:
Recombinant Protein
Size:
10ug
Species:
Mouse
Target:
LGMN
Synonyms:
Legumain
Synonyms:
Asparaginyl endopeptidase
Synonyms:
Protease
Synonyms:
cysteine 1
Source:
Sf9 Insect cells
Uniprot:
O89017
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Description

Product Name:Mouse LGMN Recombinant Protein
Product Code:RPPB1903
Size:10µg
Species:Mouse
Target:LGMN
Synonyms:Legumain,Asparaginyl endopeptidase, Protease, cysteine 1.
Source:Sf9 Insect cells
Physical Appearance:Sterile filtered colorless solution.
Formulation:LGMN protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQIIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKSGPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMMNHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVEDLTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTILKRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTAHDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAMDKVCLSHYLE HHHHHH

Legumain, also known as LGMN is a lysosomal cysteine protease which is foundin all mouse tissues, however it was mainly abundant in the kidney as well asplacenta. LGMN plays an essential role in the endosomal/lysosomal degradationsystem as the Legumain deficiency causes the accumulation of pro cathepsins B, H& L, another group of lysosomal cysteine proteases. Furthermore, overexpression of LGMN in tumors is important for invasion/metastasis.

LGMN produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 426 amino acids (18-435a.a.) andhaving a molecular mass of 48.6kDa. (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).LGMN is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.
UniProt Protein Details:
NCBI Summary:This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis. [provided by RefSeq, Aug 2016]
UniProt Code:O89017
NCBI GenInfo Identifier:7242187
NCBI Gene ID:19141
NCBI Accession:NP_035305.1
UniProt Secondary Accession:O89017
UniProt Related Accession:O89017
Molecular Weight:49,373 Da
NCBI Full Name:legumain preproprotein
NCBI Synonym Full Names:legumain
NCBI Official Symbol:Lgmn  
NCBI Official Synonym Symbols:AEP; Prsc1; AI746452; AU022324  
NCBI Protein Information:legumain
UniProt Protein Name:Legumain
UniProt Synonym Protein Names:Asparaginyl endopeptidase; Protease, cysteine 1
Protein Family:
UniProt Gene Name:Lgmn  
UniProt Entry Name:
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