Mouse LDHB Recombinant Protein (RPPB1901)
- SKU:
- RPPB1901
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Mouse
- Target:
- LDHB
- Synonyms:
- L-lactate dehydrogenase B chain
- LDH-B
- LDH heart subunit
- LDH-H
- Source:
- Escherichia Coli
- Uniprot:
- P16125
Frequently bought together:
Description
Product Name: | Mouse LDHB Recombinant Protein |
Product Code: | RPPB1901 |
Size: | 5µg |
Species: | Mouse |
Target: | LDHB |
Synonyms: | L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, LDH-H, Ldh-2, Ldh2, Ldhb. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile filtered colorless solution. |
Formulation: | LDHB protein solution (1mg/ml) containing 20mM Tris-HCl (pH8.0), 1mM DTT and 10% glycerol. |
Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
Purity: | Greater than 95.0% as determined by SDS-PAGE. |
Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL |
Biological Activity: | Specific activity is > 150 units/mg, in which one unit will 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5. |
Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
LDHB Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (1-334 a.a) and having a molecular mass of 39kDa.LDHB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
UniProt Protein Function: | |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | P16125 |
NCBI GenInfo Identifier: | 126042 |
NCBI Gene ID: | 16832 |
NCBI Accession: | P16125.2 |
UniProt Secondary Accession: | P16125,Q545Y4 |
UniProt Related Accession: | P16125 |
Molecular Weight: | 36,572 Da |
NCBI Full Name: | L-lactate dehydrogenase B chain |
NCBI Synonym Full Names: | lactate dehydrogenase B |
NCBI Official Symbol: | Ldhb |
NCBI Official Synonym Symbols: | Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582 |
NCBI Protein Information: | L-lactate dehydrogenase B chain |
UniProt Protein Name: | L-lactate dehydrogenase B chain |
UniProt Synonym Protein Names: | LDH heart subunit; LDH-H |
Protein Family: | L-lactate dehydrogenase |
UniProt Gene Name: | Ldhb |
UniProt Entry Name: |