Mouse LDHA Recombinant Protein (RPPB1894)
- SKU:
- RPPB1894
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- LDHA
- Synonyms:
- L-lactatedehydrogenase A chain
- LDH-A
- LDH muscle subunit
- LDH-M
- Source:
- Sf9 Insect cells
- Uniprot:
- P06151
Description
| Product Name: | Mouse LDHA Recombinant Protein |
| Product Code: | RPPB1894 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | LDHA |
| Synonyms: | L-lactatedehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | LDHA protein solution (0.5mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELALVDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFIIPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGVHALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEVHKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGISDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH |
| Biological Activity: | Specificactivity is > 250 units/mg, and is defined as the Amount of enzyme thatconvert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C. |
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.
LDHA Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (1-332a.a.) and having a molecular mass of 37.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).LDHA is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P06151 |
| NCBI GenInfo Identifier: | 6754524 |
| NCBI Gene ID: | 16828 |
| NCBI Accession: | NP_034829.1 |
| UniProt Secondary Accession: | P06151 |
| UniProt Related Accession: | P06151 |
| Molecular Weight: | 36,499 Da |
| NCBI Full Name: | L-lactate dehydrogenase A chain isoform 1 |
| NCBI Synonym Full Names: | lactate dehydrogenase A |
| NCBI Official Symbol: | Ldha |
| NCBI Official Synonym Symbols: | Ldh1; Ldhm; l7R2 |
| NCBI Protein Information: | L-lactate dehydrogenase A chain |
| UniProt Protein Name: | L-lactate dehydrogenase A chain |
| UniProt Synonym Protein Names: | LDH muscle subunit; LDH-M |
| Protein Family: | Leucine dehydrogenase |
| UniProt Gene Name: | Ldha |
| UniProt Entry Name: |
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