Kin of irregular chiasm-like protein 1(Kirrel1), also known as Nephrin-like protein 1(Neph1), belongs to the immunoglobulin superfamily. Kirrel1 plays a significant role in the normal development and function of the glomerular permeability. It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. The knockout of this gene could result in perinatal lethality accompanied by proteinuria, and effacement of glomerular podocytes. Kirrel1 is abundantly expressed in kidney and specifically expressed in podocytes of kidney glomeruli. Its’ subunit interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus) and interacts with NPHS1/nephrin (via the Ig-like domains). This interaction is dependent on KIRREL glycosylation. Kirrel1 also interacts when tyrosine-phosphorylated with GRB2.
Product Name:
Mouse KIRREL1/NEPH1 Recombinant Protein (RPES1247)
Product Code:
RPES1247
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Kin of IRRE-like protein 1,Kin of irregular chiasm-like protein 1,Nephrin-like protein 1,Kirrel1,Neph1
Accession:
Q80W68
Sequence:
Leu48-Leu525
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse KIRREL1 is produced by our Mammalian expression system and the target gene encoding Leu48-Leu525 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
53.4 kDa
AP Mol Mass:
60-90 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
