The Mouse ITGAM (CD11b/Integrin alpha M) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of ITGAM in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential component for a variety of research studies. ITGAM, also known as CD11b/Integrin alpha M, is a key protein involved in immune response and inflammation. Its role in various diseases such as autoimmune disorders, infectious diseases, and inflammatory conditions makes it a critical biomarker for investigating the underlying mechanisms and potential therapeutic targets.
By using the Mouse ITGAM (CD11b/Integrin alpha M) ELISA Kit, researchers can gain valuable insights into the role of ITGAM in disease pathology and develop innovative strategies for treatment and management. Order your kit today and advance your research with confidence.
Product Name:
Mouse Itgam/CD11b (Integrin alpha-M) ELISA Kit
SKU:
MOES01558
Target:
Mouse Itgam/CD11b (Integrin alpha-M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Itgam/CD11b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Itgam/CD11b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Itgam/CD11b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Itgam/CD11b. You can calculate the concentration of Mouse Itgam/CD11b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-103
84-98
89-103
Average (%)
98
91
94
1:4
Range (%)
93-105
87-100
88-102
Average (%)
99
92
94
1:8
Range (%)
90-105
84-94
81-95
Average (%)
98
89
87
1:16
Range (%)
87-99
82-93
83-98
Average (%)
92
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.06
108.27
454.33
53.28
104.62
465.14
Standard deviation
3.57
6.18
14.81
2.66
5.34
25.3
C V (%)
6.86
5.71
3.26
4.99
5.1
5.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Itgam/CD11b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Itgam/CD11b in samples. No significant cross-reactivity or interference between Mouse Itgam/CD11b and analogues was observed.
