Mouse IQ Motif Containing GTPase Activating Protein 1 (IQGAP1) ELISA Kit
The Mouse IQ Motif Containing GTPase Activating Protein 1 (IQGAP1) ELISA Kit is a valuable tool for researchers studying the role of IQGAP1 in various biological processes. IQGAP1 is a key regulator of cell signaling and adhesion, impacting cell migration, proliferation, and invasion. This kit offers exceptional sensitivity and specificity, enabling accurate quantification of IQGAP1 levels in mouse serum, plasma, and cell lysates.
IQGAP1 is implicated in numerous diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its importance as a potential therapeutic target. By measuring IQGAP1 levels, researchers can gain insights into disease mechanisms and assess the effectiveness of potential treatments. Trust the Mouse IQGAP1 ELISA Kit for precise and reliable results in your research endeavors.
Product Name:
Mouse IQ Motif Containing GTPase Activating Protein 1 (IQGAP1) ELISA Kit
Product Code:
MODL00734
Size:
96 Assays
Target:
IQGAP1
Synonyms:
HUMORFA01, SAR1, p195
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
