Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
The Mouse IP-10 (CXCL10) Interferon Gamma-Induced Protein 10kDa ELISA Kit is a powerful tool for quantitative measurement of IP-10 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this ELISA kit provides accurate and reliable results, making it an indispensable tool for researchers in the field.IP-10, also known as CXCL10, is a chemokine induced by interferon gamma and plays a critical role in immune responses, inflammation, and cell migration. Its dysregulation has been implicated in various diseases, including autoimmune disorders, viral infections, and cancer.
Therefore, the Mouse IP-10 ELISA Kit is essential for studying the role of IP-10 in these pathologies and developing potential therapeutic interventions.Overall, the Mouse IP-10 Interferon Gamma-Induced Protein 10kDa ELISA Kit from AssayGenie offers a reliable and accurate method for quantifying IP-10 levels in mouse samples, facilitating groundbreaking research in immunology, oncology, and beyond.
Product Name:
Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU:
MOES00642
Target:
Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IP-10/CXCL10. You can calculate the concentration of Mouse IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
89-106
90-103
Average (%)
93
97
97
1:4
Range (%)
95-111
84-95
98-112
Average (%)
101
89
103
1:8
Range (%)
99-113
81-95
93-110
Average (%)
106
87
100
1:16
Range (%)
94-109
86-100
91-105
Average (%)
101
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.3
250.0
728.8
87.9
251.0
683.9
Standard deviation
5.3
14.3
36.4
5.5
12.0
31.5
C V (%)
5.45
5.72
4.99
6.26
4.78
4.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed.
