Mouse Interleukin 5 Receptor Alpha (IL-5Ra) ELISA Kit

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SKU:
MODL00713
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21183
Sensitivity:
28.5pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
CDw125,CD125,IL5R-A,IL5-RA,HSIL5R3,IL5R,IL5R-A
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Mouse Interleukin 5 Receptor Alpha (IL-5Ra) ELISA Kit

The Mouse Interleukin-5 Receptor Alpha (IL5RA) ELISA Kit is a high-quality assay designed for the accurate and sensitive detection of IL5RA levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for studying the role of IL5RA in various research applications.IL5RA is a critical receptor involved in the immune response, particularly in allergic reactions and inflammatory processes.

Understanding the expression and regulation of IL5RA is crucial for uncovering its role in diseases such as asthma, allergies, and autoimmune disorders. This ELISA kit enables researchers to accurately measure IL5RA levels, providing valuable insights into its function and potential therapeutic targets.

Product Name:Mouse Interleukin 5 Receptor Alpha (IL-5Ra) ELISA Kit
Product Code:MODL00713
Size:96 Assays
Target:IL5Ra
Synonyms:CDw125, CD125, IL5R-A, IL5-RA, HSIL5R3, IL5R, IL5R-A
Detection Method:Sandwich
Range:62.5-4000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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