Mouse Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit
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- SKU:
- MODL00694
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P25085
- Sensitivity:
- 11.2pg/mL
- Range:
- 31.25-2000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- IL1RN,IL1-RA,ICIL1RA,IL1ra3,IL1F3,IRAP
- Reactivity:
- Mouse
- Sample Type:
- Serum, Plasma, Tissue Homogenates And Other Biological Fluids
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Description
system_update_altDatasheet
Mouse Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit
The Mouse Interleukin-1 Receptor Antagonist (IL-1RA) ELISA Kit is specifically designed for the precise measurement of IL-1RA levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL-1RA is an important protein that acts as a natural inhibitor of the pro-inflammatory effects of interleukin-1 (IL-1).
By blocking IL-1 receptors, IL-1RA plays a critical role in regulating the immune response and inflammation. Dysregulation of IL-1RA has been linked to various inflammatory diseases and autoimmune disorders, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.Order the Mouse IL-1RA ELISA Kit today and advance your research in immunology, inflammation, and autoimmune diseases.
| Product Name: | Mouse Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit |
| Product Code: | MODL00694 |
| Size: | 96 Assays |
| Target: | IL1RA |
| Synonyms: | IL1RN, IL1-RA, ICIL1RA, IL1ra3, IL1F3, IRAP, IL-1RA, Interleukin-1 Family Member 3, IL1 inhibitor, Anakinra |
| Detection Method: | Sandwich |
| Range: | 31.25-2000pg/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
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| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
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