Mouse Interleukin 1 Family, Member 9 (IL-1F9) ELISA Kit

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SKU:
MODL00691
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3U0P4
Sensitivity:
2.6pg/mL
Range:
7.81-500pg/mL
ELISA Type:
Sandwich
Synonyms:
IL-1F9,IL36g,IL-1H1,IL-1RP2,IL1E,IL1H1,IL1RP2,Interleukin 36, Gamma,Interleukin-1 Homolog 1,Interleukin-1 epsilon,Interleukin 1-Related Protein 2
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Mouse Interleukin 1 Family, Member 9 (IL-1F9) ELISA Kit

The Mouse Interleukin-1 Family Member 9 (IL1F9) ELISA Kit is a reliable assay designed for the detection of IL1F9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL1F9 is a member of the interleukin-1 family and plays a crucial role in inflammatory responses and immune regulation.

Its dysregulation has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse IL1F9 ELISA Kit is a valuable tool for researchers interested in studying the role of IL1F9 in various physiological and pathological processes in mouse models.

Product Name:Mouse Interleukin 1 Family, Member 9 (IL-1F9) ELISA Kit
Product Code:MODL00691
Size:96 Assays
Target:IL1F9
Synonyms:IL-1F9, IL36g, IL-1H1, IL-1RP2, IL1E, IL1H1, IL1RP2, Interleukin 36, Gamma, Interleukin-1 Homolog 1, Interleukin-1 epsilon, Interleukin 1-Related Protein 2
Detection Method:Sandwich
Range:7.81-500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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