Mouse Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

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SKU:
MODL00742
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JHH5
Sensitivity:
12.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL11,I-TAC,IP9,SCYB11,SCYB9B,B-R1,Chemokine(C-X-C-Motif)ligand 11,Small Inducible Cytokine Subfamily B Member 11,Interferon gamma-inducible protein 9
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

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Mouse Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

The Mouse Interferon-Inducible T-cell Alpha Chemoattractant (ITAC) ELISA Kit offered by AssayGenie is specifically designed for the accurate quantification of ITAC levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for a variety of research applications.ITAC is a key chemokine involved in immune responses, specifically in recruiting T-cells to sites of inflammation.

Understanding the role of ITAC in immune regulation is crucial for studying autoimmune diseases, inflammatory conditions, and assessing the effectiveness of immunotherapies.With its high-quality components and optimized protocols, the Mouse ITAC ELISA Kit from AssayGenie is an essential tool for researchers looking to investigate the role of ITAC in immune responses and inflammatory diseases.

Product Name:Mouse Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit
Product Code:MODL00742
Size:96 Assays
Target:ITaC
Synonyms:CXCL11, I-TAC, IP9, SCYB11, SCYB9B, B-R1, Chemokine(C-X-C-Motif)ligand 11, Small Inducible Cytokine Subfamily B Member 11, Interferon gamma-inducible protein 9
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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