Mouse Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) ELISA Kit
The Mouse Inositol Polyphosphate 4-Phosphatase Type I (107kDa) (INPP4A) ELISA Kit is a powerful tool for the quantitative measurement of INPP4A levels in mouse samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.INPP4A is an important enzyme involved in the regulation of inositol signaling pathways, influencing various cellular processes such as cell growth, proliferation, and survival. Dysregulation of INPP4A has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders, highlighting its significance as a potential therapeutic target and biomarker for disease progression.
By using the Mouse INPP4A ELISA Kit, researchers can gain valuable insights into the role of INPP4A in disease development and progression, providing a deeper understanding of its mechanisms and potential implications for therapeutic interventions. This kit is a valuable tool for studying inositol signaling pathways and exploring novel treatment strategies for INPP4A-related conditions.
Product Name:
Mouse Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) ELISA Kit
Product Code:
MODL00725
Size:
96 Assays
Target:
INPP4A
Synonyms:
INPP4, Inositol polyphosphate 4-phosphatase type I, Type I inositol 3,4-bisphosphate 4-phosphatase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
