The Mouse INHB (Inhibin B) CLIA Kit is a powerful tool for measuring inhibin B levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, providing accurate and consistent results for a variety of research purposes.Inhibin B is a key regulator of reproductive function and plays a crucial role in the regulation of follicle-stimulating hormone (FSH) secretion. Dysregulation of inhibin B levels has been implicated in conditions such as infertility, polycystic ovary syndrome (PCOS), and testicular dysfunction, making it a valuable biomarker for investigating these disorders and developing potential treatments.
This mouse INHB CLIA Kit is easy to use and offers rapid assay times, making it suitable for high-throughput screening and quantitative analysis. Trust assaygenie for reliable and efficient solutions for your research needs.
Product Name:
Mouse INHB (Inhibin B) CLIA Kit
SKU:
MOES00383
Target:
Mouse INHB (Inhibin B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INHB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse INHB. You can calculate the concentration of Mouse INHB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-121
86-99
100-113
Average (%)
110
93
107
1:4
Range (%)
83-99
98-112
86-96
Average (%)
90
106
91
1:8
Range (%)
94-108
86-100
97-111
Average (%)
101
93
103
1:16
Range (%)
84-97
92-107
90-101
Average (%)
90
97
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
84-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.23
45.4
168.59
22.01
48.1
172.14
Standard deviation
2.25
5.2
18.31
2.68
4.16
12.96
C V (%)
9.29
11.45
10.86
12.18
8.65
7.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse INHB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INHB in samples. No significant cross-reactivity or interference between Mouse INHB and analogues was observed.
