The Mouse IL-6 ELISA Kit is a highly sensitive and specific assay designed to accurately measure interleukin-6 levels in mouse serum, plasma, and tissue culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.Interleukin-6 is a key cytokine involved in inflammation and immune response regulation. It plays a critical role in various physiological processes, including the immune system, hematopoiesis, and acute phase responses. Abnormal levels of IL-6 have been associated with a wide range of diseases, including inflammatory disorders, autoimmune diseases, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Mouse IL-6 ELISA Kit is a valuable tool for researchers looking to investigate the role of interleukin-6 in different biological pathways and diseases in mouse models. Its high sensitivity and specificity ensure accurate and reliable results, enabling precise measurements of IL-6 levels for a better understanding of its biological functions.
Product Name:
Mouse IL-6 ELISA Kit
SKU:
MOES00663
Target:
Mouse IL-6
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
91-103
98-111
Average (%)
98
98
104
1:4
Range (%)
94-109
88-103
98-110
Average (%)
100
94
104
1:8
Range (%)
94-108
87-98
95-106
Average (%)
101
93
100
1:16
Range (%)
97-113
84-97
97-109
Average (%)
105
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.4
174.5
952.8
105.3
162.7
1040.7
Standard deviation
6.4
7.7
50.5
6.5
8.1
38.5
C V (%)
6.44
4.41
5.3
6.17
4.98
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-6 in samples. No significant cross-reactivity or interference between Mouse IL-6 and analogues was observed.
