Based on the information provided in the URL www.assaygenie.com/mouse-il34-clia-kit-moes00401/, here is a product description:The Mouse IL-34 CLIA Kit is a cutting-edge assay designed for the precise quantification of interleukin-34 (IL-34) levels in mouse samples including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL-34 is a crucial cytokine involved in the regulation of immune responses, inflammation, and tissue homeostasis.
It plays a key role in various physiological and pathological processes, making it a valuable biomarker for studying conditions such as autoimmune diseases, inflammatory disorders, and cancer. The Mouse IL-34 CLIA Kit provides researchers with a powerful tool to investigate the role of IL-34 in health and disease, ultimately leading to potential therapeutic advancements.
Product Name:
Mouse IL-34 CLIA Kit
SKU:
MOES00401
Target:
Mouse IL-34
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-34. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-34, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-34. You can calculate the concentration of Mouse IL-34 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
89-103
102-121
Average (%)
98
94
110
1:4
Range (%)
94-108
95-109
91-102
Average (%)
101
100
96
1:8
Range (%)
93-104
94-104
84-98
Average (%)
98
99
90
1:16
Range (%)
98-114
92-104
102-119
Average (%)
106
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
99-113
105
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.26
86.99
272.42
40.62
84.9
256.36
Standard deviation
3.39
8.61
28.09
3.36
6.76
22.15
C V (%)
8.02
9.9
10.31
8.27
7.96
8.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-34 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-34 in samples. No significant cross-reactivity or interference between Mouse IL-34 and analogues was observed.
