The Mouse IL-2SR Alpha (CD25) ELISA Kit is specifically designed for the precise quantification of IL-2SR Alpha (CD25) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.IL-2SR Alpha (CD25) is a critical cell surface receptor involved in the immune response, particularly in regulating T cell activation and proliferation.
Dysregulation of this receptor is linked to autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a biomarker in disease research and therapeutic development.With its advanced technology and reliable performance, the Mouse IL-2SR Alpha (CD25) ELISA Kit is an invaluable tool for studying the role of this crucial protein in immune system function, disease pathogenesis, and potential therapeutic interventions.
Product Name:
Mouse IL-2sR alpha/CD25 ELISA Kit
SKU:
MOES01490
Target:
Mouse IL-2sR alpha/CD25
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Interleukin-2 Receptor alpha chain. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Interleukin-2 Receptor alpha chain and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Interleukin-2 Receptor alpha chain, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Interleukin-2 Receptor alpha chain. You can calculate the concentration of Mouse Interleukin-2 Receptor alpha chain in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
89-104
96-109
Average (%)
99
95
103
1:4
Range (%)
92-106
83-95
89-101
Average (%)
98
89
95
1:8
Range (%)
87-98
82-92
83-93
Average (%)
92
87
88
1:16
Range (%)
91-104
85-98
83-94
Average (%)
97
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
94-109
99
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
246.98
579.84
2015.75
247.76
539.7
1990.3
Standard deviation
17.02
25.86
95.55
14.27
30.82
103.5
C V (%)
6.89
4.46
4.74
5.76
5.71
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Interleukin-2 Receptor alpha chain concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Interleukin-2 Receptor alpha chain in samples. No significant cross-reactivity or interference between Mouse Interleukin-2 Receptor alpha chain and analogues was observed.
