The Mouse IL-27 CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of IL-27 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IL-27 is a key cytokine involved in regulating immune responses, promoting anti-inflammatory effects, and modulating T cell function.
It plays a critical role in various diseases, including autoimmune disorders, infectious diseases, and inflammatory conditions, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Mouse IL-27 CLIA Kit offers researchers a powerful tool for measuring IL-27 levels accurately and efficiently, supporting advancements in immunology and drug development.
Product Name:
Mouse IL-27 CLIA Kit
SKU:
MOES00413
Target:
Mouse IL-27
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-27, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-27. You can calculate the concentration of Mouse IL-27 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
92-106
90-103
Average (%)
108
98
96
1:4
Range (%)
96-109
98-111
94-109
Average (%)
104
104
100
1:8
Range (%)
90-104
85-100
98-115
Average (%)
97
92
105
1:16
Range (%)
104-116
100-115
86-98
Average (%)
110
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
92-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.26
126.28
332.0
42.06
121.0
314.31
Standard deviation
4.16
12.06
21.81
4.76
13.92
23.6
C V (%)
9.84
9.55
6.57
11.32
11.5
7.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-27 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-27 in samples. No significant cross-reactivity or interference between Mouse IL-27 and analogues was observed.
