The Mouse IL-25 ELISA Kit is specifically designed for the precise measurement of interleukin 25 (IL-25) levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for a variety of research applications.IL-25 is a key cytokine that plays a crucial role in regulating immune responses, particularly in allergic inflammation and autoimmune diseases.
By measuring IL-25 levels, researchers can better understand the mechanisms underlying these conditions and potentially uncover new therapeutic targets.Overall, the Mouse IL-25 ELISA Kit provides researchers with a reliable and effective means to study the role of IL-25 in various disease processes and develop novel treatment strategies.
Product Name:
Mouse IL-25 ELISA Kit
SKU:
MOES00769
Target:
Mouse IL-25
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-25. You can calculate the concentration of Mouse IL-25 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
92-103
98-115
Average (%)
104
97
106
1:4
Range (%)
93-108
86-99
82-92
Average (%)
100
93
88
1:8
Range (%)
88-102
84-99
82-95
Average (%)
94
90
87
1:16
Range (%)
87-99
79-92
87-101
Average (%)
92
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.94
140.25
422.86
55.24
134.27
436.28
Standard deviation
3.49
6.68
22.79
3.65
5.63
21.16
C V (%)
6.85
4.76
5.39
6.61
4.19
4.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-25 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-25 in samples. No significant cross-reactivity or interference between Mouse IL-25 and analogues was observed.
