The Mouse IL1RA (Interleukin 1 Receptor Antagonist) ELISA Kit is a powerful tool for accurately measuring IL1RA levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and reproducible results, making it an excellent choice for a variety of research applications.IL1RA is a key regulatory protein that helps control inflammation by blocking the activity of interleukin-1 (IL-1), a pro-inflammatory cytokine. Dysregulation of IL1RA has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases.
As such, measuring IL1RA levels can provide valuable insights into the underlying mechanisms of these diseases and aid in the development of targeted therapies. The Mouse IL1RA ELISA Kit from Assay Genie is a valuable tool for researchers studying inflammation, immune responses, and related pathways in mouse models. With its precise and reliable results, this kit can help advance our understanding of IL1RA biology and its role in disease pathogenesis.
Product Name:
Mouse IL-1ra ELISA Kit
SKU:
MOES00658
Target:
Mouse IL-1ra
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1ra/IL-1F3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1ra/IL-1F3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1ra/IL-1F3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1ra/IL-1F3. You can calculate the concentration of Mouse IL-1ra/IL-1F3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
85-100
90-106
Average (%)
105
91
97
1:4
Range (%)
89-103
81-95
82-96
Average (%)
95
87
88
1:8
Range (%)
91-104
80-92
84-94
Average (%)
98
86
89
1:16
Range (%)
92-108
83-97
85-101
Average (%)
99
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.42
300.7
929.82
107.89
292.8
924.46
Standard deviation
6.99
14.13
35.05
6.08
16.81
46.32
C V (%)
6.57
4.7
3.77
5.64
5.74
5.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1ra/IL-1F3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed.
