The Mouse IL-10 ELISA Kit is specially designed for the quantitative detection of interleukin-10 (IL-10) in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.IL-10 is a key immunoregulatory cytokine that plays a crucial role in modulating immune responses and inflammation. It is involved in regulating T cell differentiation, B cell function, and macrophage activation.
Dysregulation of IL-10 has been linked to various autoimmune diseases, inflammatory disorders, and infections, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Mouse IL-10 ELISA Kit provides researchers with a powerful tool for studying the role of IL-10 in immune responses and inflammation, offering valuable insights for advancing our understanding of disease mechanisms and developing novel treatment approaches.
Product Name:
Mouse IL-10 ELISA Kit
SKU:
MOES00665
Target:
Mouse IL-10
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-10. You can calculate the concentration of Mouse IL-10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
96-111
90-102
Average (%)
93
102
95
1:4
Range (%)
95-111
82-94
95-109
Average (%)
102
88
101
1:8
Range (%)
98-116
82-95
92-104
Average (%)
106
89
98
1:16
Range (%)
102-117
85-97
98-112
Average (%)
108
91
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51
125.6
414.8
53.2
121.4
406.8
Standard deviation
2.7
6.0
12.4
3.6
6.2
18.7
C V (%)
5.29
4.78
2.99
6.77
5.11
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-10 in samples. No significant cross-reactivity or interference between Mouse IL-10 and analogues was observed.
