The Mouse IL-1 Beta ELISA Kit is specifically designed for the precise measurement of interleukin-1 beta levels in mouse serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and consistent results, making it perfect for a variety of research applications.Interleukin-1 beta is a key cytokine that plays a crucial role in inflammation and immune response regulation.
It is involved in various physiological and pathological processes, including infection, autoimmune diseases, and inflammatory conditions. This ELISA kit allows researchers to study the role of interleukin-1 beta in disease progression and develop potential therapeutic strategies.
Product Name:
Mouse IL-1 beta ELISA Kit
SKU:
MOES00657
Target:
Mouse IL-1 beta
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1β. You can calculate the concentration of Mouse IL-1β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
96-107
85-99
Average (%)
95
101
92
1:4
Range (%)
98-112
86-98
92-109
Average (%)
105
93
100
1:8
Range (%)
98-116
86-99
93-105
Average (%)
106
91
100
1:16
Range (%)
98-113
85-97
91-104
Average (%)
107
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
96
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.4
59.3
201.2
27.3
57.8
199.4
Standard deviation
1.7
3.2
10.1
1.8
2.4
8.8
C V (%)
6.44
5.4
5.02
6.59
4.15
4.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1β in samples. No significant cross-reactivity or interference between Mouse IL-1β and analogues was observed.
