Mouse IL 5 Recombinant Protein (RPPB0517)
- SKU:
- RPPB0517
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- IL 5
- Synonyms:
- EDF
- BCDFII
- TRF
- T-cell replacing factor
- Source:
- Escherichia Coli
- Uniprot:
- P04401
Description
| Product Name: | Mouse IL 5 Recombinant Protein |
| Product Code: | RPPB0517 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL 5 |
| Synonyms: | EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor II, IL-5, BCGF-II, Cytotoxic T-lymphocyte inducer, Il5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated (1mg/ml) solution containing 20mM Sodium Phosphate and 50mM NaCl pH-7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized Mouse Interleikin-5 in sterile 18M?-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Mouse Interleukin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL5 should be stored at 4°C between 2-7 days and for future use below -18°C.Please avoid freeze thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG |
| Biological Activity: | The ED50 as determined by the dose-dependant stimulation of the proliferation of TF-1 cells was found to be 1.7ng/ml, corresponding to a specific activity of 588,235.3IU/mg. |
The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Interleukin-5 Mouse Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 2 x 113 amino acids forming a disulfide linked homodimer and having a molecular mass of 26.2 kDa.The Mouse IL-5 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cytokine Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-5 receptor binding; growth factor activity; cytokine activity Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; positive regulation of eosinophil differentiation |
| UniProt Code: | P04401 |
| NCBI GenInfo Identifier: | 124342 |
| NCBI Gene ID: | 16191 |
| NCBI Accession: | P04401.1 |
| UniProt Related Accession: | P04401 |
| Molecular Weight: | 15,410 Da |
| NCBI Full Name: | Interleukin-5 |
| NCBI Synonym Full Names: | interleukin 5 |
| NCBI Official Symbol: | Il5 |
| NCBI Official Synonym Symbols: | Il-5 |
| NCBI Protein Information: | interleukin-5; TRF; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor |
| UniProt Protein Name: | Interleukin-5 |
| UniProt Synonym Protein Names: | B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il5 |
| UniProt Entry Name: | IL5_MOUSE |
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