Mouse IL 33 Recombinant Protein (RPPB0665)
- SKU:
- RPPB0665
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- IL 33
- Synonyms:
- Interleukin 33
- DVS27
- NF-HEV
- NKHEV
- Source:
- Escherichia Coli
- Uniprot:
- Q8BVZ5
Description
| Product Name: | Mouse IL 33 Recombinant Protein |
| Product Code: | RPPB0665 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL 33 |
| Synonyms: | Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, Il-33, Il1f11, 9230117N10Rik, Il33. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Mouse IL-33 was lyophilized from a sterile 0.2 micron filtered aqueous solution containing 10mM sodium phosphate, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized IL-33 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL-33 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-33 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSIQGTSLLT QSPASLSTYN DQSVSFVLEN GCYVINVDDS GKDQEQDQVL LRYYESPCPA SQSGDGVDGK KLMVNMSPIK DTDIWLHAND KDYSVELQRG DVSPPEQAFF VLHKKSSDFV SFECKNLPGT YIGVKDNQLA LVEEKDESCN NIMFKLSKI |
| Biological Activity: | The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is 0.04ng/ml, corresponding to a specific activity of 2.5x107units/mg. |
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
Interleukin33 Mouse recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids and having a molecular mass of 17.7 kDa.
| UniProt Protein Function: | IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine Cellular Component: extracellular space; extracellular region; chromosome; cytoplasmic vesicle; nucleus Molecular Function:cytokine activity Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; defense response to virus; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response |
| UniProt Code: | Q8BVZ5 |
| NCBI GenInfo Identifier: | 68565902 |
| NCBI Gene ID: | 77125 |
| NCBI Accession: | Q8BVZ5.1 |
| UniProt Secondary Accession: | Q8BVZ5,Q2YEJ4, Q3TQN0, Q99L46, |
| UniProt Related Accession: | Q8BVZ5 |
| Molecular Weight: | 29,991 Da |
| NCBI Full Name: | Interleukin-33 |
| NCBI Synonym Full Names: | interleukin 33 |
| NCBI Official Symbol: | Il33 |
| NCBI Official Synonym Symbols: | Il-33; Il1f11; NF-HEV; 9230117N10Rik |
| NCBI Protein Information: | interleukin-33; nuclear factor from high endothelial venules |
| UniProt Protein Name: | Interleukin-33 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il33 |
| UniProt Entry Name: | IL33_MOUSE |
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