Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
Product Name:
Mouse IL-23 Recombinant Protein (RPES1303)
Product Code:
RPES1303
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
SGRF,IL-23p19,CLMF p40,IL-12 subunit p40,NKSF2
Accession:
Q9EQ14&P43432
Sequence:
Val22-Ala196&Met23-Ser335
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Interleukin-23 is produced by our Mammalian expression system and the target geneencoding Val22-Ala196&Met23-Ser335 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
9.7&36.8 kDa
AP Mol Mass:
40-55 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
