Mouse IL 22 Recombinant Protein (RPPB0641)
- SKU:
- RPPB0641
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- IL 22
- Synonyms:
- IL-TIF
- TIFa
- IL-10-related T-cell-derived-inducible factor
- IL-22
- Source:
- Escherichia Coli
- Uniprot:
- Q9JJY9
Description
| Product Name: | Mouse IL 22 Recombinant Protein |
| Product Code: | RPPB0641 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL 22 |
| Synonyms: | IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated solution at 0.65mg/ml containing 0.003mM NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized pegylated mouse Interleukin -22 in sterile 0.4% NaHCO3 adjusted to pH 8-9 not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized pegylated murine IL22 although stable at room temperature for several days, should be stored desiccated below -20?C. Upon reconstitution at 0.1mg/ml pegylated mouse IL22 and up to 2mg/ml, filter and sterilized, the protein can be stored at 4 degrees Celsius for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE. |
| Biological Activity: | The ED50 as determined by STAT3 phosphorylation assay in HepG cells. The activity in vitro was found to be ~ 10% compared to the non-pegylated mouse IL22. |
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acids signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10RBeta (previously known as CRF2-4), belonging to the class II cytokine recep
Pegylated Interleukin-22 Mouse Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and an aditional Ala amino acid at N-terminus having a molecular mass of 36 kDa as determioned by mass spectometry. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as a 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein.The Murine IL-22 is Mono-pegylated (with 20 kDa PEG) purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cytokine; Secreted Cellular Component: extracellular space; extracellular region Molecular Function:protein binding; cytokine activity Biological Process: regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | Q9JJY9 |
| NCBI GenInfo Identifier: | 17366895 |
| NCBI Gene ID: | 50929 |
| NCBI Accession: | Q9JJY9.1 |
| UniProt Secondary Accession: | Q9JJY9,Q14BB3, |
| UniProt Related Accession: | Q9JJY9 |
| Molecular Weight: | 20,106 Da |
| NCBI Full Name: | Interleukin-22 |
| NCBI Synonym Full Names: | interleukin 22 |
| NCBI Official Symbol: | Il22 |
| NCBI Official Synonym Symbols: | IL-22; Iltif; IL-22a; ILTIFa |
| NCBI Protein Information: | interleukin-22; ILTIF alpha; IL-TIF alpha; interleukin-22a; IL-10-related T-cell-derived-inducible factor; interleukin 10-related T cell-derived inducible factor |
| UniProt Protein Name: | Interleukin-22 |
| UniProt Synonym Protein Names: | IL-10-related T-cell-derived-inducible factor; IL-TIF; IL-TIF alpha; Interleukin-22a; IL-22a |
| UniProt Gene Name: | Il22 |
| UniProt Entry Name: | IL22_MOUSE |
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