The Mouse IL-10 Monoclonal Antibody (FITC-conjugated, JES5-16E3, AGEL2030) from Assay Genie is an essential tool for researchers studying interleukin-10 (IL-10), a key immunoregulatory cytokine with anti-inflammatory properties. This monoclonal antibody, labeled with FITC for fluorescence applications, is specifically designed for detecting and monitoring IL-10 expression in mouse samples.IL-10 is a cytokine that plays a crucial role in immune regulation and tolerance, making it a significant target for studies in immunology, inflammation, and autoimmune diseases. The FITC-conjugated monoclonal antibody allows for precise identification and quantification of IL-10 levels in various experimental settings, enabling researchers to investigate the role of this important cytokine in different biological processes.
Ideal for flow cytometry, immunofluorescence, and other fluorescent-based assays, this antibody provides reliable and consistent results, aiding in the accurate analysis of IL-10 expression and function in mouse models. With its high specificity and sensitivity, the Mouse IL-10 Monoclonal Antibody (FITC-conjugated) is a valuable tool for understanding the role of IL-10 in immune responses and disease pathogenesis.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P18893
Gene ID:
16153
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
L-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.