The Mouse IgG2a (Immunoglobulin G2a) ELISA Kit is specifically designed for the accurate quantification of IgG2a levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an excellent tool for various research applications.Immunoglobulin G2a is an important antibody subclass involved in immune responses, particularly in antibody-mediated immunity.
Detection and measurement of IgG2a levels are essential for studying immune responses, monitoring disease progression, and evaluating potential treatments in various experimental models.Overall, the Mouse IgG2a ELISA Kit provides researchers with a reliable and efficient method for quantifying IgG2a levels in mouse samples, facilitating a better understanding of immune responses and immunological processes in research studies.
Product Name:
Mouse IgG2a (Immunoglobulin G2a) ELISA Kit
SKU:
MOES01191
Target:
Mouse IgG2a (Immunoglobulin G2a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG2a. You can calculate the concentration of Mouse IgG2a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
97-111
93-110
Average (%)
96
103
100
1:4
Range (%)
88-99
85-95
85-95
Average (%)
94
90
90
1:8
Range (%)
92-104
84-97
83-97
Average (%)
98
89
89
1:16
Range (%)
86-100
80-91
86-98
Average (%)
93
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
91-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.93
53.22
216.02
27.87
56.33
215.11
Standard deviation
1.79
3.11
6.85
1.82
3.05
10.13
C V (%)
6.65
5.84
3.17
6.53
5.41
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgG2a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG2a in samples. No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed.
