The Mouse IgG (Immunoglobulin G) ELISA Kit, available at www.assaygenie.com/mouse-igg-immunoglobulin-g-elisa-kit-moes01189/, is a reliable and accurate tool for quantifying mouse IgG levels in various biological samples. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it ideal for a wide range of research applications.Immunoglobulin G, or IgG, is a key component of the immune system and plays a crucial role in immune responses against pathogens.
By measuring IgG levels, researchers can gain valuable insights into the immune status and response of experimental animals. This ELISA kit offers a convenient and efficient way to study the humoral immune response in mouse models, aiding in the understanding of various diseases and conditions.Overall, the Mouse IgG ELISA Kit is a valuable tool for researchers working in immunology, infectious diseases, and translational medicine, providing accurate and reliable data for their studies.
Product Name:
Mouse IgG (Immunoglobulin G) ELISA Kit
SKU:
MOES01189
Target:
Mouse IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG. You can calculate the concentration of Mouse IgG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
87-101
89-106
Average (%)
106
93
97
1:4
Range (%)
95-106
86-101
90-106
Average (%)
101
93
97
1:8
Range (%)
100-115
84-98
95-111
Average (%)
107
90
103
1:16
Range (%)
98-111
83-93
95-111
Average (%)
105
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.5
11.3
34.7
5.4
11.7
34.3
Standard deviation
0.4
0.5
1.8
0.3
0.6
1.2
C V (%)
7.27
4.42
5.19
5.56
5.13
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG in samples. No significant cross-reactivity or interference between Mouse IgG and analogues was observed.
