The Mouse IgG Fc ELISA Kit is specifically designed for the accurate and reliable detection of mouse IgG Fc levels in a variety of samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a range of research applications.IgG Fc is a critical component of the immune system, playing a key role in antibody-mediated responses to pathogens and foreign substances. Monitoring IgG Fc levels can provide valuable insights into immune function, inflammation, and autoimmune disorders, making this kit indispensable for studies in immunology, infectious diseases, and drug development.
With its user-friendly protocol and robust performance, the Mouse IgG Fc ELISA Kit is an essential tool for researchers seeking to accurately measure and analyze mouse IgG Fc levels in their experimental samples. Order now to elevate your research to the next level.
Product Name:
Mouse IgG-Fc ELISA Kit
SKU:
MOES01186
Target:
Mouse IgG-Fc
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Fcγ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Fcγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Fcγ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Fcγ. You can calculate the concentration of Mouse Fcγ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
89-101
98-112
Average (%)
92
95
103
1:4
Range (%)
87-102
84-98
87-99
Average (%)
94
90
94
1:8
Range (%)
90-103
83-96
84-96
Average (%)
96
89
90
1:16
Range (%)
91-107
81-94
84-95
Average (%)
99
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.53
47.63
142.96
21.07
42.9
154.93
Standard deviation
1.12
2.16
4.92
1.14
2.32
6.06
C V (%)
5.73
4.53
3.44
5.41
5.41
3.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Fcγ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Fcγ in samples. No significant cross-reactivity or interference between Mouse Fcγ and analogues was observed.
