Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
The Mouse IGFBP5 (Insulin-like Growth Factor Binding Protein 5) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of IGFBP5 levels in mouse serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it suitable for a variety of research applications.IGFBP5 is a key regulator of insulin-like growth factor signaling, influencing cell growth, survival, and differentiation.
It plays a crucial role in various physiological processes and has been implicated in diseases such as cancer, diabetes, and osteoporosis. The Mouse IGFBP5 ELISA Kit is a valuable tool for studying the role of IGFBP5 in these conditions and exploring potential therapeutic interventions.
Product Name:
Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
SKU:
MOES01739
Target:
Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGFBP-5. You can calculate the concentration of Mouse IGFBP-5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
95-108
89-100
Average (%)
105
101
94
1:4
Range (%)
93-107
87-99
88-103
Average (%)
99
92
95
1:8
Range (%)
91-103
86-97
85-95
Average (%)
98
92
89
1:16
Range (%)
91-101
85-97
84-99
Average (%)
96
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
403.2
775.71
3305.74
376.19
779.46
3571.63
Standard deviation
24.64
41.89
174.21
19.37
42.79
122.51
C V (%)
6.11
5.4
5.27
5.15
5.49
3.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IGFBP-5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-5 in samples. No significant cross-reactivity or interference between Mouse IGFBP-5 and analogues was observed.
