Mouse IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) CLIA Kit
The Mouse IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) CLIA Kit is a reliable tool for measuring levels of IGFBP-4 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for various research applications.IGFBP-4 is a key regulator of insulin-like growth factor signaling, playing a crucial role in cell growth, differentiation, and survival. Its dysregulation has been associated with several diseases, including cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
For researchers looking to investigate the role of IGFBP-4 in mouse models or cell cultures, the Mouse IGFBP-4 CLIA Kit provides a convenient and efficient solution with its user-friendly protocol and precise quantification capabilities. Unlock the potential of your research with this innovative kit from Assay Genie.
Product Name:
Mouse IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) CLIA Kit
SKU:
MOES00391
Target:
Mouse IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGFBP-4. You can calculate the concentration of Mouse IGFBP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
91-106
90-103
Average (%)
99
98
97
1:4
Range (%)
91-103
96-113
103-120
Average (%)
97
103
110
1:8
Range (%)
91-105
103-117
95-106
Average (%)
97
108
101
1:16
Range (%)
92-104
91-107
93-106
Average (%)
99
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
425.78
788.87
3433.82
455.22
767.11
3515.46
Standard deviation
40.92
79.12
336.51
56.67
87.3
247.14
C V (%)
9.61
10.03
9.8
12.45
11.38
7.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGFBP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-4 in samples. No significant cross-reactivity or interference between Mouse IGFBP-4 and analogues was observed.
