Mouse IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2) CLIA Kit
The Mouse IGFBP-2 (Insulin-like Growth Factor Binding Protein 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IGFBP-2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.IGFBP-2 is a key protein involved in regulating insulin-like growth factor signaling and is known to play a role in cell growth, differentiation, and survival.
Abnormal levels of IGFBP-2 have been linked to various diseases such as cancer, diabetes, and cardiovascular disorders, making it an important biomarker for studying these conditions and exploring potential therapeutic strategies.With its high-quality components and user-friendly format, the Mouse IGFBP-2 CLIA Kit is a valuable tool for researchers looking to investigate the role of IGFBP-2 in health and disease.
Product Name:
Mouse IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2) CLIA Kit
SKU:
MOES00390
Target:
Mouse IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGFBP-2. You can calculate the concentration of Mouse IGFBP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
92-106
89-104
Average (%)
90
98
95
1:4
Range (%)
95-107
84-96
98-110
Average (%)
100
91
103
1:8
Range (%)
86-98
93-105
92-107
Average (%)
93
98
98
1:16
Range (%)
94-106
85-99
99-117
Average (%)
101
92
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
99-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.71
519.17
1631.89
172.08
569.45
1744.85
Standard deviation
18.3
38.89
125.66
16.26
54.5
141.86
C V (%)
9.96
7.49
7.7
9.45
9.57
8.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGFBP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-2 in samples. No significant cross-reactivity or interference between Mouse IGFBP-2 and analogues was observed.
