Mouse IGF-2 (Insulin Like Growth Factor 2) CLIA Kit
The Mouse IGF-2 (Insulin-like Growth Factor 2) CLIA Kit is a highly reliable and accurate tool for detecting IGF-2 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with precise and reproducible results, making it suitable for a variety of research applications.IGF-2, a member of the insulin-like growth factor family, plays a crucial role in cell growth, proliferation, and differentiation. Abnormal levels of IGF-2 have been associated with various diseases, including cancer, diabetes, and growth disorders.
By accurately measuring IGF-2 levels, researchers can gain valuable insights into the mechanisms underlying these conditions, paving the way for potential therapeutic interventions.Overall, the Mouse IGF-2 CLIA Kit is a valuable tool for studying the role of IGF-2 in health and disease, offering researchers a reliable and sensitive method for detecting and measuring this important growth factor in mouse samples.
Product Name:
Mouse IGF-2 (Insulin Like Growth Factor 2) CLIA Kit
SKU:
MOES00388
Target:
Mouse IGF-2 (Insulin Like Growth Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGF-2. You can calculate the concentration of Mouse IGF-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
96-112
100-116
Average (%)
92
103
106
1:4
Range (%)
93-110
89-105
89-102
Average (%)
101
96
96
1:8
Range (%)
90-103
100-114
99-113
Average (%)
95
106
106
1:16
Range (%)
84-99
98-111
98-113
Average (%)
91
104
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
107
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.53
97.75
334.21
42.12
105.63
343.68
Standard deviation
4.29
7.01
31.68
4.81
10.53
38.84
C V (%)
10.85
7.17
9.48
11.42
9.97
11.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGF-2 in samples. No significant cross-reactivity or interference between Mouse IGF-2 and analogues was observed.
