The Mouse IGF-1 (Insulin-like Growth Factor 1) CLIA Kit is a highly reliable and sensitive assay designed for the accurate quantification of IGF-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional specificity and precision, ensuring consistent and trustworthy results for various research applications.IGF-1 is a vital growth factor that plays a critical role in regulating cell growth, proliferation, and differentiation. It is involved in various physiological processes, including muscle development, bone growth, and metabolism.
Dysregulation of IGF-1 has been linked to conditions such as diabetes, cancer, and aging, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its high-performance capabilities and ease of use, the Mouse IGF-1 CLIA Kit from Assay Genie is an essential tool for researchers seeking to accurately measure IGF-1 levels in mouse samples for a deeper understanding of biological processes and disease mechanisms.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGF-1. You can calculate the concentration of Mouse IGF-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
94-106
90-105
Average (%)
100
101
96
1:4
Range (%)
97-112
85-98
96-112
Average (%)
104
92
102
1:8
Range (%)
100-115
92-103
92-105
Average (%)
108
97
99
1:16
Range (%)
89-102
102-116
87-100
Average (%)
96
110
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
92-106
97
Cell culture media (n=5)
91-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.43
119.15
356.5
49.44
111.09
360.41
Standard deviation
5.08
10.07
23.92
5.91
8.53
33.7
C V (%)
9.33
8.45
6.71
11.95
7.68
9.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGF-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGF-1 in samples. No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed.
