The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 ( interferon gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interteron receptor while IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-γ was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-γ exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types.
Product Name:
Mouse IFNGR1/CD119 Recombinant Protein (RPES1923)
Product Code:
RPES1923
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
CD119,Ifgr,IFN-gammaR,Ifngr,Nktar
Accession:
P15261
Sequence:
Met 1-Asp 253
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA.Immobilized mouse IFNG-His at 10 µg/ml (100 ?l/well) can bind mouse IFNGR1-Fc. The EC50 of IFNGR1-Fc is 58.2-135.9 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse IFNGR1 (P15261) extracellular domain (Met 1-Asp 253) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
53 kDa
AP Mol Mass:
70-75 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
