The Mouse HSP-90 (Heat Shock Protein 90) ELISA Kit is specifically designed for the precise measurement of HSP-90 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results, making it an excellent choice for various research applications.HSP-90 is a key molecular chaperone that plays a critical role in protein folding and cell signaling. It is involved in cellular responses to stress and is implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases.
Monitoring HSP-90 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Mouse HSP-90 ELISA Kit is a valuable tool for researchers looking to study the role of HSP-90 in health and disease, offering reliable and reproducible data for advancing their research goals.
Product Name:
Mouse HSP-90 (Heat Shock Protein 90) ELISA Kit
SKU:
MOES01128
Target:
Mouse HSP-90 (Heat Shock Protein 90)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP-90. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HSP-90. You can calculate the concentration of Mouse HSP-90 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
97-111
99-114
Average (%)
98
105
105
1:4
Range (%)
100-115
87-102
95-110
Average (%)
105
93
103
1:8
Range (%)
100-114
83-98
94-105
Average (%)
107
89
99
1:16
Range (%)
97-114
85-100
96-112
Average (%)
105
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
98
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.0
4.8
0.5
0.9
4.4
Standard deviation
0.03
0.04
0.25
0.03
0.04
0.22
C V (%)
6
4.0
5.21
6.0
4.44
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HSP-90 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HSP-90 in samples. No significant cross-reactivity or interference between Mouse HSP-90 and analogues was observed.
