The Mouse HNF4A (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit is specifically designed for the accurate quantification of HNF4A levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.HNF4A is a key transcription factor involved in the regulation of genes essential for liver development, function, and metabolism.
Dysregulation of HNF4A has been linked to various liver disorders, making it a valuable biomarker for studying hepatic diseases and potential therapeutic interventions.With easy-to-follow protocols and a user-friendly format, the Mouse HNF4A ELISA Kit is an essential tool for researchers studying liver biology, metabolism, and related diseases in mouse models. Get accurate and reliable data to advance your research with confidence.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HNF4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HNF4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HNF4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HNF4a. You can calculate the concentration of Mouse HNF4a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
95-109
98-112
Average (%)
102
100
103
1:4
Range (%)
87-102
85-99
83-96
Average (%)
94
91
89
1:8
Range (%)
87-101
78-92
83-93
Average (%)
93
85
87
1:16
Range (%)
87-100
81-94
83-92
Average (%)
93
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
126.81
348.8
1053.83
137.37
338.03
978.8
Standard deviation
7.19
17.89
33.72
7.32
17.81
29.56
C V (%)
5.67
5.13
3.2
5.33
5.27
3.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HNF4a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HNF4a in samples. No significant cross-reactivity or interference between Mouse HNF4a and analogues was observed.
