Mouse HMGB-1 (High mobility group protein B1) CLIA Kit
The Mouse HMGB-1 (High Mobility Group Protein B1) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of HMGB-1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an excellent tool for a variety of research applications.HMGB-1 is a key protein involved in inflammation, immune response, and cell death regulation. It plays a crucial role in conditions such as sepsis, arthritis, and autoimmune diseases, making it a valuable biomarker for studying these diseases and developing new treatment strategies.
Overall, the Mouse HMGB-1 CLIA Kit offers researchers a reliable and efficient method for measuring HMGB-1 levels in mouse samples, helping to advance our understanding of HMGB-1 biology and its significance in various disease processes.
Product Name:
Mouse HMGB-1 (High mobility group protein B1) CLIA Kit
SKU:
MOES00369
Target:
Mouse HMGB-1 (High mobility group protein B1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
98-114
97-114
Average (%)
93
105
104
1:4
Range (%)
100-115
100-111
103-119
Average (%)
109
105
109
1:8
Range (%)
85-99
97-107
85-100
Average (%)
91
102
92
1:16
Range (%)
89-103
86-97
88-102
Average (%)
96
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.08
64.7
219.31
25.6
62.53
206.96
Standard deviation
2.4
5.23
13.33
2.91
4.69
22.77
C V (%)
9.97
8.08
6.08
11.37
7.5
11.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HMGB-1 in samples. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed.
