The Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit is specifically designed for the accurate and reliable detection of HDAC2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.HDAC2 is a key enzyme involved in regulating gene expression by removing acetyl groups from histone proteins, playing a critical role in chromatin remodeling and gene transcription. Dysregulation of HDAC2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, making it a valuable biomarker for studying disease mechanisms and developing potential therapeutic interventions.
With easy-to-follow protocols and rapid assay times, the Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit provides researchers with a powerful tool for investigating the role of HDAC2 in health and disease, advancing our understanding of epigenetic regulation and potential treatment strategies.
Product Name:
Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit
Product Code:
MODL00610
Size:
96 Assays
Target:
HDAC2
Synonyms:
RPD3, YAF1, HD2
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
