Based on the provided URL, the Mouse Hepcidin ELISA Kit from Assay Genie is a powerful tool for detecting levels of hepcidin in mouse samples. Hepcidin is a key regulator of iron metabolism, playing a crucial role in conditions such as anemia, hemochromatosis, and iron deficiency disorders.This kit offers high sensitivity and specificity, ensuring accurate and reliable results in serum, plasma, and cell culture supernatants.
By measuring hepcidin levels, researchers can gain valuable insights into iron regulation and its impact on various health conditions.The Mouse Hepcidin ELISA Kit from Assay Genie is essential for studying iron metabolism and its implications for health and disease. Get reliable and reproducible results for your research with this advanced kit.
Product Name:
Mouse Hepc (Hepcidin) ELISA Kit
SKU:
MOES01173
Target:
Mouse Hepc (Hepcidin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Hepc. You can calculate the concentration of Mouse Hepc in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
98-115
87-99
Average (%)
101
105
93
1:4
Range (%)
98-110
82-95
99-113
Average (%)
104
87
105
1:8
Range (%)
98-116
82-93
92-106
Average (%)
106
88
100
1:16
Range (%)
100-114
87-98
94-107
Average (%)
107
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
89-103
97
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
385
686.4
3381.6
352.7
696.9
3600.6
Standard deviation
23.5
30.2
121.7
24.7
38.3
144.0
C V (%)
6.1
4.4
3.6
7.0
5.5
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Hepc concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Hepc in samples. No significant cross-reactivity or interference between Mouse Hepc and analogues was observed.
