Mouse Heparan Sulfate Proteoglycan 2 (HSPG2) ELISA Kit

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SKU:
MODL00647
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q05793
Sensitivity:
32.4pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
PLC,PRCAN,SJA,SJS,SJS1,Perlecan,Endorepellin,Schwartz-Jampel Syndrome 2,Basement membrane-specific heparan sulfate proteoglycan core protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Mouse Heparan Sulfate Proteoglycan 2 (HSPG2) ELISA Kit

The Mouse Heparan Sulfate Proteoglycan 2 (HSPG2) ELISA Kit is specifically designed for the accurate detection of HSPG2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.HSPG2 is a critical component in extracellular matrix structure and functions, playing a key role in cell adhesion, migration, and differentiation.

Dysregulation of HSPG2 has been implicated in various diseases including cancer, cardiovascular disorders, and renal diseases, making it a valuable biomarker for studying disease mechanisms and developing potential therapeutic interventions.With the Mouse HSPG2 ELISA Kit, researchers can confidently analyze HSPG2 levels in mouse samples with ease and accuracy, providing valuable insights into the role of HSPG2 in health and disease.

Product Name:Mouse Heparan Sulfate Proteoglycan 2 (HSPG2) ELISA Kit
Product Code:MODL00647
Size:96 Assays
Target:HSPG2
Synonyms:PLC, PRCAN, SJA, SJS, SJS1, Perlecan, Endorepellin, Schwartz-Jampel Syndrome 1, Basement membrane-specific heparan sulfate proteoglycan core protein
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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