Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit

(No reviews yet) Write a Review
SKU:
MODL00638
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q80Y52
Sensitivity:
31pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
HSP86,HSP90A,HSP90N,HSPC1,HSPCA,HSPCAL1,HSPCAL4,HSPN,Hsp89,Hsp90,LAP2,Lipopolysaccharide-associated protein 2,Renal carcinoma antigen NY-REN-38
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit

The Mouse Heat Shock Protein 90kDa Alpha A1 (Hsp90aa1) ELISA Kit is a reliable and precise tool for measuring levels of Hsp90aa1 in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are essential for a wide range of research applications.Hsp90aa1 is a key molecular chaperone involved in protein folding and quality control, playing a critical role in cellular homeostasis and stress response.

Dysregulation of Hsp90aa1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. This ELISA kit is an invaluable resource for studying Hsp90aa1 function and exploring its potential as a therapeutic target.

Product Name:Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit
Product Code:MODL00638
Size:96 Assays
Target:HSP90aA1
Synonyms:HSP86, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

0 Reviews

View AllClose

Related Products