Mouse Heat Shock 60kD Protein 1, Chaperonin (HSPD1) ELISA Kit
SKU:
MODL00646
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P63038
Sensitivity:
0.11ng/mL
Range:
0.234-15ng/mL
ELISA Type:
Sandwich
Synonyms:
HSP60,GROEL,CPN60,HuCHA60,Heat Shock Protein 60,Spastic Paraplegia 13,Autosomal Dominant,60 kDa chaperonin,Mitochondrial matrix protein P1,P61 lymphocyte protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Mouse Heat Shock 60kD Protein 1, Chaperonin (HSPD1) ELISA Kit
The Mouse Heat Shock 60kD Protein 1 (Chaperonin HSPD1) ELISA Kit from Assaygenie is specifically designed for the accurate detection of HSPD1 levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for various research applications.HSPD1, also known as Heat Shock Protein 60 (HSP60), is a chaperonin protein involved in protein folding and quality control. It plays a crucial role in maintaining cellular homeostasis and protecting cells from stress-induced damage.
Dysregulation of HSPD1 has been linked to various diseases such as cancer, neurodegenerative disorders, and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Mouse HSPD1 ELISA Kit from Assaygenie is a valuable tool for researchers looking to study the role of HSPD1 in health and disease, providing accurate and reliable measurements for their experiments.
Product Name:
Mouse Heat Shock 60kD Protein 1, Chaperonin (HSPD1) ELISA Kit
Product Code:
MODL00646
Size:
96 Assays
Target:
HSPD1
Synonyms:
HSP60, GROEL, CPN60, HuCHA60, Heat Shock Protein 60, Spastic Paraplegia 13,Autosomal Dominant, 60 kDa chaperonin, Mitochondrial matrix protein P1, P60 lymphocyte protein
Detection Method:
Sandwich
Range:
0.234-15ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
