The Mouse HDL (High Density Lipoprotein) ELISA Kit is specially designed for the precise measurement of HDL levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, which makes it perfect for various research purposes.HDL is an important component involved in lipid metabolism, playing a vital role in cardiovascular health by removing excess cholesterol from the bloodstream.
Monitoring HDL levels is crucial in understanding the risk of heart disease and other related conditions. This ELISA kit provides a reliable method for studying HDL levels in mouse models, offering valuable insights into lipid metabolism and potential therapeutic strategies.
Product Name:
Mouse HDL (High Density Lipoprotein) ELISA Kit
SKU:
MOES01676
Target:
Mouse HDL (High Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
1.88 nmol/mL
Detection range:
3.13-200 nmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HDL. You can calculate the concentration of Mouse HDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
88-100
90-103
Average (%)
98
94
96
1:4
Range (%)
89-104
87-99
82-94
Average (%)
96
92
88
1:8
Range (%)
91-104
84-97
85-101
Average (%)
96
91
92
1:16
Range (%)
91-107
85-97
90-104
Average (%)
99
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
91-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.27
26.46
80.09
9.66
27.63
78.73
Standard deviation
0.7
1.38
2.96
0.5
1.12
3.13
C V (%)
6.82
5.22
3.7
5.18
4.05
3.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HDL in samples. No significant cross-reactivity or interference between Mouse HDL and analogues was observed.
