The Mouse HB (Hemoglobin) CLIA Kit is specifically designed for the detection of hemoglobin levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in various research applications.Hemoglobin is a key protein responsible for oxygen transport in the blood, playing a crucial role in maintaining physiological functions. Abnormalities in hemoglobin levels can indicate various health conditions such as anemia, cardiovascular diseases, and respiratory disorders, making it a valuable biomarker for disease monitoring and research studies.
With its advanced technology and user-friendly protocol, the Mouse HB CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating hemoglobin levels in mouse models, contributing to a better understanding of hematological disorders and potential therapeutic strategies.
Product Name:
Mouse HB (Hemoglobin) CLIA Kit
SKU:
MOES00300
Target:
Mouse HB (Hemoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HB. You can calculate the concentration of Mouse HB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
83-95
91-102
Average (%)
110
90
97
1:4
Range (%)
100-111
88-103
90-104
Average (%)
105
95
96
1:8
Range (%)
90-103
93-107
88-99
Average (%)
97
100
94
1:16
Range (%)
83-98
92-108
100-112
Average (%)
90
98
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.7
11.22
29.64
4.02
10.33
26.81
Standard deviation
0.45
0.8
1.93
0.36
0.77
1.99
C V (%)
12.16
7.13
6.51
8.96
7.45
7.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HB in samples. No significant cross-reactivity or interference between Mouse HB and analogues was observed.
