The Mouse H3 Histone (Histone H3) ELISA Kit is specifically designed for the quantitative detection of Mouse H3 Histone in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Histone H3 is a key protein involved in chromatin structure and gene regulation, playing a critical role in epigenetic processes such as DNA replication, repair, and transcription. Abnormalities in histone H3 levels have been linked to various diseases, including cancer, metabolic disorders, and neurological conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
Overall, the Mouse H3 Histone ELISA Kit provides researchers with a powerful tool for investigating the role of histone H3 in health and disease, offering a precise and efficient method for measuring its levels in biological samples.
Product Name:
Mouse H3 (Histone H3) ELISA Kit
SKU:
MOES00960
Target:
Mouse H3 (Histone H3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse H3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse H3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse H3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse H3. You can calculate the concentration of Mouse H3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
97-112
94-105
Average (%)
93
103
100
1:4
Range (%)
91-105
83-96
82-96
Average (%)
99
89
88
1:8
Range (%)
89-102
87-100
84-97
Average (%)
94
92
88
1:16
Range (%)
88-101
82-94
87-100
Average (%)
95
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.24
4.86
0.53
1.35
5.32
Standard deviation
0.03
0.07
0.16
0.04
0.07
0.28
C V (%)
5.88
5.65
3.29
7.55
5.19
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse H3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse H3 in samples. No significant cross-reactivity or interference between Mouse H3 and analogues was observed.
