The Mouse GT Gastrin ELISA Kit is specifically developed for the precise measurement of GT Gastrin levels in mouse samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it suitable for various research purposes. GT Gastrin is a key hormone involved in regulating gastric acid secretion and stimulating cell growth in the stomach lining. It plays a crucial role in gastrointestinal health and is implicated in conditions such as peptic ulcers and gastrinomas.
Studying GT Gastrin levels can provide valuable insights into these conditions and aid in the development of potential treatments. With its reliable performance and ease of use, the Mouse GT Gastrin ELISA Kit is an essential tool for researchers studying the role of GT Gastrin in gastric physiology and associated disorders.
Product Name:
Mouse GT (Gastrin) ELISA Kit
SKU:
MOES01804
Target:
Mouse GT (Gastrin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GT. During the reaction, GT in the sample or standard competes with a fixed amount of GT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
97-115
87-100
Average (%)
97
105
94
1:4
Range (%)
86-101
91-107
96-113
Average (%)
93
98
103
1:8
Range (%)
87-99
89-105
98-112
Average (%)
92
96
106
1:16
Range (%)
86-99
91-105
92-107
Average (%)
91
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.5
139.0
440.9
44.2
146.3
406.4
Standard deviation
3
7.5
23.4
2.9
8.6
14.6
C V (%)
6.19
5.4
5.31
6.56
5.88
3.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes GT in samples. No significant cross-reactivity or interference between GT and analogues was observed.
